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Rockland Immunochemicals offers streptavidin and avidin conjugated to the fluorochrome AMCA to be used with any biotinylated antibody or probe to introduce a bright blue fluorescence staining to cells.
AMCA provides an excellent additional fluorochrome in multilabeling experiments because this dye has virtually no overlap with other traditionally employed fluorochromes. Rockland uses 7-amino-3-((((succinimidyl)oxy)carbonyl)methyl)-4-methylcoumarin-6-sulfonic acid obtained from Molecular Probes Inc. Use a standard UV filter set for photography. Certain films which are blue sensitive may further enhance the detection of fluorescent staining using this dye. Inquire for AMCA conjugates to any other proteins.
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: firstname.lastname@example.orgPhone: 800.656.7625