Seeing is believing. You can literally see the quality of Rockland antibodies using a variety of techniques including immunofluorescence microscopy. Visual evidence of proper target localization and low background staining is immediately apparent when cells are screened after staining. Protect your experiments with Rockland antibodies. Compromise elsewhere.  

1. Background 



Visualizing biological samples using a fluorescent microscope and antibodies conjugated to fluorescent labels is a powerful technique to localize structures within tissues, cells or subcellular compartments. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. Immunofluorescence microscopy is a widely used example of immunostaining and is a form of immunohistochemistry based on the use of fluorophores to visualize the location of bound antibodies.




Immunofluorescence can be used on tissue sections, cultured cells or individual cells that are fixed by a variety of methods. Antibodies can be used in this method to analyze the distribution of proteins, glycoproteins and other antigen targets including small biological and non-biological molecules.



2. Types of Fluorescent Microscopes
3. Assay Formats for Immunofluorescence Microscopy
4. List of Fluorescent Labels Used for Antibody Conjugation by Rockland
5. ATTO-TEC Fluorescent Dye Conjugates
6. CyDye® Fluorescent Dye Conjugates
7. Dylight™ Fluorescent Dye Conjugates
8. IRDye® Fluorescent Dye Conjugates
9. Other Fluorescent Dye Conjugates