Immunofluorescence Microscopy - Fluorescent Labels


Immunofluorescence Microscopy - 4. Fluorescent Labels Used for Antibody Conjugation by Rockland



 

Fluorescent label

 

 

Color

 

 

Abs (nm)

 

Em (nm) MW (daltons)

 

DyLight™ 405

Violet

400

420

793

Aminomethylcoumarin (AMCA)

Violet Blue

353

442

410

ATTO 425

Blue

436

484

498

Cy2™

Blue Green

489

505

897

DyLight™ 488

Blue Green

493

518

1,011

ATTO 488

Green

501

523

981

Fluorescein (FITC)

Green

495

528

390

ATTO 532

Yellow Green

532

553

1081

Cy3™

Yellow Green

552

565

949

DyLight™ 549

Yellow Green

550

568

982

Rhodamine (TRITC)

Orange

550

570

444

R-Phycoerythrin (RPE)

Orange

488

575

240,000

ATTO 550

Orange

554

576

791

Cy3.5™

Orange Red

581

596

1,286

Texas Red®

Red

596

620

625

ATTO 594

Red

601

627

1389

Allophycocyanin

Far-Red

650

660

100,000

Cy5™

Far-Red

650

667

975

ATTO 647N

Far-Red

644

669

843

DyLight™ 649

Far-Red

646

674

1,008

ATTO 655

Far-Red

663

684

887

IRDye®700DX

Near Infra-Red

689

700

1,954

Cy5.5™

Near Infra-Red

678

703

1,312

DyLight™ 680

Near Infra-Red

682

715

950

DyLight™ 800

Infra-Red

770

794

1,050

IRDye® 800CW 

Infra-Red

774

800

1,166

IRDye® 800

Infra-Red

778

806

1,166



Immunofluorescence Microscopy

  1. Background
  2. Types of Fluorescent Microscopes
  3. Assay Formats for Immunofluorescence Microscopy
  4. List of Fluorescent Labels Used for Antibody Conjugation by Rockland
  5. ATTO-TEC Fluorescent Dye Conjugates
  6. CyDye® Fluorescent Dye Conjugates
  7. Dylight™ Fluorescent Dye Conjugates
  8. IRDye® Fluorescent Dye Conjugates
  9. Other Fluorescent Dye Conjugates