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Nuclear Extract PDF
The protocol for the isolation of nuclear and cytoplasmic fractions can be used to generate material for the gel shift/gel super shift assays or any other assay requiring similar source material. Remember to use only specially prepared reagents that are free of any contaminating proteases or nucleases and to keep the preparation on ice where indicated. A common troubleshooting finding is that breakdown products caused by non-specific proteolysis are recognized in systems typically as lower molecular weight bands.
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