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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

Green Fluorescent Protein (GFP) Control Protein

Code

000-001-215
Size

100 µg
Price

$295.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western Blot of anti-GFP monoclonal antibody.  
Lane 1:  64pg of recombinant GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364).  
Lane 2:  32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Lane 3:  16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Lane 4:  8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Lane 5:  4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Lane 6:  2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Lane 7:  1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Lane 8:  0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. 
Primary antibody:  anti-GFP monoclonal antibody at 1:400 for overnight at 4°C.
Secondary antibody:  HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4°C. 
Block:  TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C.
Predicted/Observed size:  27 kDa for GFP. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: rGFP, GFP fusion protein

Green Fluorescent Protein (GFP) Control Protein Properties

Description
Recombinant Green Fluorescent Protein (GFP) Control Protein - 000-001-215
Application
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
n/a
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Lot#  

Green Fluorescent Protein (GFP) Control Protein Description

Background
Green Fluorescent Protein (GFP) is used as a positive control when detecting GFP fusion proteins in anti-GFP antibody assays. Polyclonal anti-GFP can detect GFP by ELISA (sandwich or capture) or western blot. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution when used in combination with monoclonal anti-GFP (p/n 600-301-215), using either form of the antibody as the capture or detection antibody. Control GFP can be used as a control in western blots. Researchers should determine optimal titers for applications.
Storage Condition
Store GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
GFP is suitable as a control for polyclonal or monoclonal anti-GFP in immunological assays. The molecular weight of GFP is 28 kDa.
Purity/Specifity
This product was expressed in E.coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Goat anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 95% pure.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
GFP and Epitopes;
Applications
ELISA, FLISA, WB, IF
Reacts With
wt, rGFP, eGFP
Catalog Number
200-341-215

Product Type
GFP and Epitopes;
Applications
IF
Reacts With
wt, rGFP, eGFP
Catalog Number
600-100-215

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
wt, rGFP, eGFP
Catalog Number
600-101-215

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry