SDS PAGE Results of Bovine IgG F(ab) Fragment

Datasheet

Bovine IgG Fab

Name: Bovine IgG Fab
Brand: Rockland Immunochemicals
Price: 210.0 USD
Availability: InStock

Item No. 001-0105

Size: 2 mg

Applications: SDS-PAGE

Origin: Bovine

$210.00 /Per Item

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Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

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Specifications for Bovine IgG Fab

Product Details

Description: Bovine IgG Fab Fragment - 001-0105

Synonyms: Bovine IgG Fab fragment

Species of Origin: Bovine

Format: IgG Fab

Type: Native Protein

Target Details

Purity/Specificity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(ab’)2. No reaction was observed against anti-Bovine IgG F(c) or anti- Papain.

Application Details

Tested Applications: SDS-PAGE

Application Note: Bovine IgG F(ab) has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation

Physical State: Liquid

Concentration: 2.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.