SDS-PAGE of Mouse IgG F(c) Fragment (p/n 010-0103)

Datasheet

Mouse IgG F(c)

Name: Mouse IgG F(c)
Brand: Rockland Immunochemicals
Price: 259.0 USD
Availability: InStock

2 References

Item No. 010-0103

Size: 1 mg

Applications: IF, SDS-PAGE, Microarray, Multiplex

Origin: Mouse

$259.00 /Per Item

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Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

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Specifications for Mouse IgG F(c)

Product Details

Description: Mouse IgG Fc Fragment - 010-0103

Synonyms: MOUSE IgG F(c) fragment, Mouse Fc, Immunoglobulin Fc

Species of Origin: Mouse

Format: IgG Fc

Type: Native Protein

Target Details

Purity/Specificity: MOUSE IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(c). No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: IF, Microarray, Multiplex - View References

Application Note: Mouse IgG F(c) Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.954 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG Fc fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Bucukovski, J et al. (2015). A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology. PloS One

Applications

Microarray, Microarray chip

PubMed

Sasakura Y et al. (2004). Protein microarray system for detecting protein-protein interactions using an anti-His-tag antibody and fluorescence scanning: effects of the heme redox state on protein-protein interactions of heme-regulated phosphodiesterase from Escherichia coli. Anal Chem.

Applications

IF, Confocal Microscopy; Multiplex Assay

PubMed

Related Protocols

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.