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GFP and Epitopes  >  Antibodies for Signal Enhancement
 

Luciferase Antibody

Goat Polyclonal
Code

100-101-150
Size

2 mL
Price

$185.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Goat anti-Luciferase Antibody.  
Lane 1:  Luciferase (Firefly).
Load:  50 ng per lane.
Primary antibody:  Goat anti-Luciferase Antibody at 1:1,000 overnight at 4°C.
Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  60 kDa, 60 kDa for Luciferase.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms:

Luciferase Antibody Properties

Description
Anti-LUCIFERASE (GOAT) Antibody - 100-101-150
Host
Goat
Known Cross Reactivity
Firefly
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Other Dilution: User Optimized
Epitope Tag Type
LUCIFERASE
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
82 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

Luciferase Antibody Description

Immunogen
Luciferase [Firefly]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
This product has been assayed against 1.0 ug of Luciferase [Firefly] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Purity/Specifity
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Firefly].  No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
S. H. Windahl, M. K. Lagerquist, N. Andersson, C. Jochems, A. Kallkopf, C. Håkansson, J. Inzunza, J.-Å. Gustafsson, P. T. van der Saag, H. Carlsten, K. Pettersson, and C. Ohlsson. (2007) Identification of Target Cells for the Genomic Effects of Estrogens in Bone. Endocrinology 148(12):5688–5695.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry