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Primary Antibodies  >  Glucose Energy Metabolism Antibodies
 

Glucose Oxidase Antibody

Goat Polyclonal
Code

100-1137
Size

2 mL
Price

$309.00
Availability

Ships next business day.
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Western Blot of Goat anti Glucose Oxidase antibody.  
Lane 1:  ~1ug Glucose Oxidase Reduced.  
Lane 2:  ~0.25ug Glucose Oxidase Reduced.  
Lane 3:  ~1ug Glucose Oxidase Non-Reduced.
Lane 4:  ~0.25ug Glucose Oxidase Non-Reduced.  
Primary antibody:  Glucose Oxidase antibody at 1:5000 for overnight at 4°C.
Secondary antibody:  Dylight 649 conjugated Donkey anti Goat (605-743-125 lot 20834 1:10K 1.5 hr RT.
Block:  MB-070 overnight at 4°C.
Predicted/Observed size:  65.6 kDa, 90 kDa for Glucose Oxidase. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Glucose Oxidase Antibody Properties

Description
Anti-GLUCOSE OXIDASE (GOAT) Antibody - 100-1137
Host
Goat
Target Species
Aspergillus niger
Known Cross Reactivity
Aspergillus niger
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
gox
Download CofA
Lot#  

Glucose Oxidase Antibody Description

Background
Glucose oxidase antibody is specific to glucose oxidase enzyme. Glucose oxidase is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-d-lactone. Glucose oxidase is commonly used for the determination of free glucose in body fluids (diagnostics), and in the food industry. It also has many applications in biotechnologies, for example enzyme assays for biochemistry including biosensors in nanotechnologies. Glucose oxidase is often extracted from Aspergillus niger.
Immunogen
Glucose Oxidase [Aspergillus niger]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-glucose oxidase antibody has been assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Optimal concentration should be determined by the researcher.
Purity/Specifity
Anti-glucose oxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Oxidase [Aspergillus niger].  Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Julio Raba and Horacio A. Mottola (1995). "Glucose Oxidase as an Analytical Reagent". Critical Reviews in Analytical Chemistry 25 (1): 1–42. DOI:10.1080/10408349508050556
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Aspergillus niger
Catalog Number
100-4137

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Aspergillus niger
Catalog Number
100-6137

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Aspergillus niger
Catalog Number
200-101-037

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Goat
Catalog Number
605-4313

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry