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Cyclin A Antibody

Rabbit Polyclonal
NCI Collaboration
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Immunohistochemistry of Rabbit Anti-Cyclin A antibody.  
Tissue:  human testes tissue.  
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  Cyclin A antibody  at 1:500 for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  Cyclin A is nuclear and cytoplasmic.
Staining:  Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.
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Number:
100-401-151
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Cyclin A Antibody Properties

Description
Anti-Cyclin A (RABBIT) Antibody - 100-401-151
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CCNA2
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Cyclin A Antibody Description

Background
Anti-Cyclin A detects cyclin a. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-cyclin-a Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Synonyms
CCN1 antibody, CCNA antibody, CCNA2 antibody, Cyclin A2 antibody
Immunogen
Anti-Cyclin-A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Anti-Cyclin-A antibody is suitable for the detection by immunoblot, ELISA and IP of human, rat and mouse Cyclin A.  Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
Anti-Cyclin A was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 60 kDa Cyclin A protein from human, rat and mouse tissue.  No reaction was observed against other related cyclins.  Cross reactivity with Cyclin A from other species may also occur.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A. (2012) Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death and Differentiation 1-10. Nature
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat, mouse
Catalog Number
100-401-152

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-161

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Reacts With
Rabbit
Catalog Number
611-143-002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry