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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Cyclin E Antibody

Rabbit Polyclonal
Code

100-401-156
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: CCNE antibody, CCNE1 antibody, Cyclin E1 antibody, Cyclin Es antibody, Cyclin Et antibody, CyclinE antibody, G1/S specific cyclin E antibody

Cyclin E Antibody Properties

Description
Anti-Cyclin E (RABBIT) Antibody - 100-401-156
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human
Clonality
Polyclonal
Application
ELISA : 1:100,000
Western Blot : 1:2,000 - 1:10,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
70 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CCNE1
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Lot#  

Cyclin E Antibody Description

Background
Anti-Cyclin-E antibody detects cyclin-E. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-cyclin-e Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Immunogen
Anti-Cyclin-E Antibody was produced by repeated immunizations with a KLH conjugated peptide corresponding to a region near the carboxy terminus of human Cyclin E.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Anti-Cyclin-E antibody is suitable for the detection by immunoblot, ELISA and IP. Researchers should determine optimal titers for applications that are not stated below. Antiserum will specifically react with a 45 kDa Cyclin E protein from human tissue.
Purity/Specifity
Anti-Cyclin-E was prepared from monospecific antiserum by delipidation and defibrination.  No reaction was observed against other related cyclins.  Cross reactivity with Cyclin E from other species may also occur.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Koff, A., Cross, F., Fisher, A., Schumaker, J., Leguellec, K., Philippe, M. and Roberts, J.M. (1991) Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell 66; 1217-1228. Koff, A., Giordano, A., Desai, D., Yamashita, K., Harper, J.W., Elledge, S., Nishimoto, T., Morgan, D.O., Franza, B.R. and Roberts, M. (1992) Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science 257; 1689-1694. Claudio, P.P., De Luca, A., Howard, C.M., Baldi, A., Firpo, E.J., Koff, A., Paggi, M.G. and Giordano, A. (1996) Functional analysis of pRb/p130 intereaction with cyclins. Cancer Res. 56; 2003-2008.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat, mouse
Catalog Number
100-401-152

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human
Catalog Number
200-301-461

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Primary Antibodies;
Applications
ELISA, WB
Catalog Number
K911

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry