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Primary Antibodies > Cell Cycle Protein Antibodies

Cyclin T1 Antibody

Rabbit Polyclonal

Code

100-401-158

Size

100 µL

Price

$294.00

Availability

Ships next business day.

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Amino acids are numbered on the bottom of each protein. The cyclin box is indicated. Human cyclins T2a and T2b have 642 amino acids in common but different carboxyl termini (black boxes). Background Information: Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.

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Synonyms: cyclin-T1 antibody, CYCT 1 antibody, cycT1 antibody, HIVE1 antibody, Human immunodeficiency virus 1 expression antibody

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References
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Cyclin T1 Antibody Properties

Description

Anti-Cyclin T1 (RABBIT) Antibody - 100-401-158

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human, mouse, rat

Clonality

Polyclonal

Application

ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:200 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

Antiserum

Label

Unconjugated

Concentration

75 mg/mL by Refractometry

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

CCNT1

Cyclin T1 Antibody Description

Background

Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.

Immunogen

Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting and other immunological methods requiring high titer and specificity. Anti-Cyclin T1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin T1. Use paraffin embedded tissue for immunohistochemistry. HeLa cells may be used as a positive control.

Purity/Specifity

This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with an 85 kDa cyclin T1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with cyclin T1 from other species may also occur.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Bagella, L., MacLachlan, T.K., Buono, R.J., Pisano, M.M., Giordano, A. and De Luca, A. (1998) Cloning of murine cdk9/PITALRE and its tissue-specific expression in development. J. Cell Physiol. 177(2):206-13. MacLachlan, T.K., Sang, N., De Luca, A.., Puri, P.L., Levrero, M. and Giordano, A. and (1998) Binding of cdk9 to TRAF2. J. Cell Biochem. 71(4):467-478. Peng, J., Zhu, Y., Milton, J.T. and Price, D.H. (1998). Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12(5):755-762. De Falco, G. and Giordano, A. (1998) CDK( (PITALRE): A Multifunctional cdc-2-related Kinase. J. Cell. Physiol. 177; 501-506. Fujinaga, K., Cujec, T.P., Peng, J., Garriga, J., Price, D.H., Graña, X. and Peterlin, B.M. (1998) The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1 and Tat. J. Virol. 72(9) 7154-7159.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra