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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

cdk2 Antibody

Rabbit Polyclonal
Code

100-401-161
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Rabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ?g of lysate was separated on a 15% SDS-PAGE gel.  Cdk-2 is indicated by an arrowhead as a 32-33 kDa band.  Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody.  Primary antibody was reacted at room temperature for 1 h.  After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development.
Rockland's Anti-CDK2 antibody was diluted 1:500 to detect CDK2 in human skin tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Synonyms: Cdc2 related protein kinase antibody, Cdk 2 antibody, CDK2 antibody, Cell division kinase 2 antibody, Cell division protein kinase 2 antibody, Cyclin dependent kinase 2 antibody, p33 protein kinase antibody

cdk2 Antibody Properties

Description
Anti-cdk2 (RABBIT) Antibody - 100-401-161
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, rat and mouse
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:200 - 1:1,000
Immunochemistry: 1:200 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDK2
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Lot#  

cdk2 Antibody Description

Background
Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Immunogen
Anti-cdk2 antbody was prepared from whole rabbit serum by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Anti-cdk2 antbody is suitable for immunohistochemistry, western blotting and other antibody based assays.  Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry.  Optimal titers for other applications should be determined by the researcher.
Purity/Specifity
Anti-cdk2 antbody was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue.  No reaction was observed against other related cyclin dependent kinases.  Cross reactivity with cdk2 from other species may also occur.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Elledge, S.J. and Spotswood, M. (1991) A new human p34 protein kinase CDK2, identified by complementation of a cdc28 mutation, is a homolog of Xenopus Eg1.  EMBO J. 10; 2653-2659. Giordano, A., Lee, J.H., Scheppler, J.A., Herrmann, C., Harlow, E., Deuschle, U., Beach, D. and Franza, B. Jr. (1991)  Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A.  Science 253; 1271-1275. Tsai, L.H., Harlow, E. and Myerson, M. (1991)  Isolation of the human CDK2 gene that encodes the cyclin A and adenovirus E1A-associated p33 kinase.  Nature 353; 174-177.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-162

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-167

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human
Catalog Number
200-301-461

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mammalian, avian
Catalog Number
600-401-940

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry