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Primary Antibodies  >  Cancer Research Antibodies
 

c-Myc Antibody

Rabbit Polyclonal
Code

100-401-224
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Anti-c-myc is shown to detect c-myc in infected cells (lanes 2-4) showing a 66 kDa band.  No band specific staining is observed in uninfected cells (lane 1).  Detection occurs using a 1:250 dilution of antibody followed by 1:2,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.  Film exposure approximately 1’.  Other detection systems will yield similar results.
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Synonyms: Myc proto-oncogene protein antibody, Proto-oncogene c-Myc antibody, Transcription factor p64 antibody, Class E basic helix-loop-helix protein 39 antibody

c-Myc Antibody Properties

Description
Anti-c-myc (RABBIT) Antibody - 100-401-224
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Myc, c-myc
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Lot#  

c-Myc Antibody Description

Background
c-myc is involved in the control of cell proliferation and differentiation at the transition from S --> G1 phase for a normal cell.  Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (k light chain); or 22 (l light chain) is described in Burkitt's lymphoma and other B-cell lympho-proliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.  Abnormal expression can take many forms including transduction, insertional activation, translocation, and amplification.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with c-myc peptide corresponding to amino acids KHKLEQLRNSGA which map to the last 12 amino acids of the mouse
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody reacts with human and mouse c-myc protein, which is a nuclear protein with a molecular weight of approximately 66 kDa.  This antiserum detects c-myc by immunoblotting assays in both overexpressed and endogenous systems.   Reactivity in other immunoassays has not yet been fully determined although perinuclear and cytoplasmic staining has been reported.  The recommended positive controls are SK-BR-3 cells (adenocarcinoma, breast, malignant pleural effusion, human).
Purity/Specifity
This antiserum is directed against human c-myc and is useful in determining its presence in various assays. Anti-c-myc is highly specific for c-myc and shows no cross-reaction with N-myc or L-myc proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bártová, E. et al. (2000) Nuclear topography of the c-myc gene in human leukemic cells Gene 244:1-11.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-301-A37

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, human, rat, bovine, dog, macaque, opossum
Catalog Number
600-401-A37

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry