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Primary Antibodies  >  Neuroscience Antibodies
 

Dab1 Antibody

Rabbit Polyclonal
Code

100-401-225
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Analysis of GFP-Dab1 and endogenous Dab1 levels in transfected retinal cells and retinal tissue.  Western blot analysis of whole cell lysates prepared from primary retinal cultures transfected with GFP-Dab1-E (lane 1), -L (lane 2), control untransfected retinal cultures (lane 3), and retinal tissue at ED5 (lane 4), ED7 (lane 5) and ED10 (lane 6). Proteins were electrophoresed through an SDS-8% polyacrylamide gel and transferred to nitrocellulose. The membrane was immuno-stained with Rockland Immunochemical's anti-Dab1 antibody, which recognizes both GFP-Dab1 (indicated by asterisks) and endogenous forms of Dab1 (indicated by a line).   See Katval et al (2007) for additional details.
Rockland Immunochemical's anti-Dab1 is shown to detect Dab1 present in wt mouse brain extracts (lane 1). No staining is noted in similar extracts from a dab knock-out mouse (lane 2).  Detection of an 80 kDa band (arrowhead) occurs using a 1:5,000 dilution of the antibody in 1% milk in TTBS for 1 h at room temperature followed by a 1:5,000 dilution of HRP Goat-a-Rabbit with ECL visualization.  Film exposure was ~1’.  Other detection systems will yield similar results.         Storage Conditions:   Store vial at -20° C prior to opening. For extended storage, aliquot contents and freeze at -20° C.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
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Synonyms: Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody

Dab1 Antibody Properties

Description
Anti-Dab1 (400-555) (RABBIT) Antibody - 100-401-225
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:5,000
ImmunoPrecipitation: 1 µl per 500 µg
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
DAB1
Download CofA
Lot#  

Dab1 Antibody Description

Background
Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'.  Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes.  Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development.  Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain.  Cloning of human Dab1 and sequence determinations suggest a 96% identity to the mouse sequence.  Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins.  Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2.  In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Dab1 antibody is suitable for the detection of Dab1 by immunoprecipitation, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate.  For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature.  For immunoprecipitation use 1 µl of antiserum per 500 µg of brain lysate.  Perform immunoprecipitation at 4°C for 2 h.  For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 µg/ml aprotinin and 10 µg/ml leupeptin.
Purity/Specifity
Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.  This antibody is directed against Dab1 from mouse.  Cross-reactivity with other species has not been determined.  No reaction occurs with human or mouse Dab2.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
D'Arcangelo, G. et al. (1999) Reelin is a ligand for lipoprotein receptors. Neuron 24(2): 471-479. Howell, et al. (1997) Neuronal position in the developing brain is regulated by mouse disabled-1. Nature 389:733-737. Lambert deRouvroit, C. and Goffinet, A.M. (1998) Cloning of human DAB1 and mapping to chromosome 1p31-p32. Genomics 53:246-247.
Specific Reference
Katyal, S., Gao, Z., Monckton, E., Glubrecht, D. and Godbout, R. (2007) Hierarchical Disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation. J.Mol. Bio. 368; 349-364. Sheldon, M., Rice, D.S. D'Arcangelo, G., Yoneshima, H., Nakajima, K., Mikoshiba, K., Howell, B.W., Cooper, J.A., Goldowitz, D. and Curran, T. (1997) Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice. Nature 389:730-733. Keshvara,L., Magdaleno,S., Benhayon,D. and Curran,T. (2002) Cyclin-dependent kinase 5 phosphorylates disabled 1 independently of Reelin signaling. J. Neurosci. 22 (12), 4869-4877.
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B99

Product Type
Primary Antibodies;
Applications
WB, IHC
Reacts With
human
Catalog Number
209-403-B99

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, mouse, chimpanzee, rat
Catalog Number
600-401-959

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry