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Primary Antibodies  >  Neuroscience Antibodies
 

Cox-2 Antibody

Rabbit Polyclonal
Code

100-401-226
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Rockland Immuno-chemicals’ anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1ß induced WISH cell extract (lane 2).  Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.  Film exposure approximately 1’.  Other detection systems will yield similar results.
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Synonyms: Prostaglandin G/H synthase 2 antibody, Cyclooxygenase-2 antibody, Prostaglandin-endoperoxide synthase 2 antibody, Prostaglandin H2 synthase 2 antibody, PGH synthase 2 antibody, PGHS-2 antibody

Cox-2 Antibody Properties

Description
Anti-COX-2 (RABBIT) Antibody - 100-401-226
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:5,000 - 1:10,000
Immunochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
55 mg/mL by Refractometry
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PTGS2, COX2
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Lot#  

Cox-2 Antibody Description

Background
COX-2, also known as prostaglandin H synthase, was identified less than a decade ago.  Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases.   Two different isoforms of the enzyme are known, Cox-1 and  Cox and Cox-2.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human Cox-2.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression.  This antiserum against Human Cox-2 and has been tested for use in immunoblotting and ELISA.  The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b.  Reactivity in other immunoassays is unknown.
Purity/Specifity
This antiserum is directed against human Cox-2 and is useful in determining its presence in western blotting and ELISA experiments.  The antibody does not cross react with Cox-1.  Cox-2 is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression.  Given its role in synthesizing prostaglandins, Cox-2 is therefore of interest in studying immune response regulation.  This antibody can detect Cox-2 in human, mouse and rat.   Reactivity of this antibody with Cox-2 from other species is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Hittelman, A.B. et al., (1999). Differential regulation of glucocorticoid receptor transcriptional activation via AF-1- associated proteins. EMBO J Oct 1;18(19):5380-8. Gierse, J. K. et al. (1996) A Single Amino Acid Difference Between Cyclooxygenase-1 (COX-1) and -2 (COX-2) Reverses the Selectivity of COX-2 Specific Inhibitors. J. Biol. Chem. 271:15810-15814.
Specific Reference
Tai, H. et. al., (2000) Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells. Biochem Biophys Res Commun. Sep 16;276(1):77-81. Guan, Z. et al. (1998) Interleukin-1-induced Cyclooxygenase-2 Expression Requires Activation of Both c-Jun NH2-terminal Kinase and p38 MAPK Signal Pathways in Rat Renal Mesangial Cells. J.Biol Chem, 273(44): 28670-28676.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry