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Primary Antibodies  >  Transcription Factor Antibodies
 

DRIP130 Antibody

Rabbit Polyclonal
Code

100-401-239
Size

500 µL
Price

$309.00
Availability

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Anti-DRIP-130 is shown to immunoprecipitate 35S-labeled in vitro translated human DRIP130 (lane 1).  A similar precipitation was performed for pre-immune serum (lane 2). Immunoprecipitation occurs using a  1:100 to 1:200 dilution of the antiserum. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background.
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Synonyms: Mediator of RNA polymerase II transcription subunit 23, Mediator complex subunit 23 Cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3 Transcriptional coactivator CRSP130 ARC130, DRIP130, KIAA1216, SUR2

DRIP130 Antibody Properties

Description
Anti-DRIP130 (RABBIT) Antibody - 100-401-239
Host
Rabbit
Target Species
Human
Known Cross Reactivity
mouse, human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:1,000
ImmunoPrecipitation: 1:100 - 1:200
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
MED23, DRIP130
Download CofA
Lot#  

DRIP130 Antibody Description

Background
This antiserum recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130), and was tested for use in immunoprecipitation and immunoblotting.  DRIP-130 has been described elsewhere as Sur2; CRSP130, ARC130, NAT140, and TRAP150b.    DRIP-130 is component of a multi-protein complex termed DRIP that interacts with the vitamin D receptor (VDR); together, the DRIP proteins constitute a new cofactor complex.  DRIP binds to several nuclear receptors and mediates ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of two other cofactor complexes called ARC and TRAP, which are recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates.  Several DRIP/ARC/TRAP subunits are also components of other potentially related cofactors, such as CRSP, NAT, SMCC and the mouse Mediator, indicating that unique classes of activators may share common sets or subsets of cofactors. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues  897-916 (based on human DRIP-130 residue numbering)  crosslinked to KLH.
Immunogen Type
Peptide
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested; pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for applications should be determined by the researcher. Rockland Immunochemical's anti-DRIP130 has been used to detect DRIP130 using nuclear extracts from mouse thymus, spleen, and brain. Unfortunately, we have not tested reactivity on any cell line extracts. The following standard procedure is recommended: separate 10 micrograms of thymus nuclear extract by 7.5% SDS-PAGE (0.75 to 1 mm thick). Transfer to nitrocellulose without SDS in the transfer buffer (pre-equilibrate the gel in transfer buffer for 30 min prior to transfer). Blocking is performed with 5% non-fat dry milk in TTBS for 1 hr at room temperature. Incubate anti-DRIP130 antibody at a 1:1,000 for 1 hr at room temperature. Dilute Donkey anti-Rabbit IgG-HRP 1:5,000 and react 1 hr at room temperature. A predominant band at ~130 KDa is detected under these conditions. Control or normal rabbit serum fails to recognize the 130 kDa protein.
Purity/Specifity
This antiserum was processed by delipidation and defibrination and is directed against mouse DRIP130.  This reagent can be used to determine the presence of DRIP130 various immunological assays. Cross-reactivity occurs with human DRIP-130.  Reactivity with DRIP-130 isolated from other species is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Boyer, T.G., et al. (1999). Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein. Nature May 20;399(6733):276-9. Rachez, C. et al. (1999). Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature Apr 29;398(6730):824-8. Ko, L. et al., (2000). Thyroid receptor-binding protein, an LXXLL motif containing protein, functions as a general coactivator. Proc Natl Acad Sci USA May 23;97(11):6212-7.
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IHC, IF
Catalog Number
K505

Product Type
Molecular Biology Reagents;
Catalog Number
MB-015

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry