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Primary Antibodies  >  Protease and Transaminase Antibodies
 

Protective Protein/Cathepsin A Antibody

Rabbit Polyclonal
Code

100-401-395
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Rockland's Anti-PPCA antibody was diluted 1:500 to detect PPCA in human brain cortex tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Pulse-chase labeling. COS-1 cells were transfected with cDNAs encoding neuraminidase and PPCA, labeled with [3H]leucine for 1 h and chased with cold leucine for the time periods indicated. Cell lysates and medium samples were immunoprecipitated with anti-PPCA antiserum. Exposure was for 20 days.  This antibody immunoprecipitates 3 forms of PPCA:  a 54 kDa precursor, and both the 32 kDa and 20 kDa mature subunits.  Personal Communication, E. Bonten.  See van der Spoel et al (1998) for additional details.
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Synonyms: Beta Galactosidase Protective Protein antibody, Carboxypeptidase C antibody, Cathepsin A antibody, CTSA antibody, EC 3.4.16.5 antibody, Glactosialidosis antibody, GLB2 antibody, Goldberg Syndrome antibody

Protective Protein/Cathepsin A Antibody Properties

Description
Anti-Protective Protein/Cathepsin A (PPCA) (RABBIT) Antibody - 100-401-395
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
CTSA, PPGB
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Lot#  

Protective Protein/Cathepsin A Antibody Description

Background
Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with native 54-kDa human Protective Protein/Cathepsin A (PPCA) precursor, purified from the medium of baculovirus infected insect cells.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody is suitable for the detection of human PPCA (54-, 32-, and 20-kDa) on Western blot, by immunoprecipitation, immunocytochemistry, and ELISA of either transfected cells (e.g. COS-1 cells) or primary cells (e.g. fibroblasts).
Purity/Specifity
This antiserum is directed against PPCA and reacts with the PPCA from human tissues.  Based on sequence we expect this antibody to react with PPCA from other sources, although specific reactivity has not been confirmed.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Cuervo AM, Mann L, Bonten EJ, d'Azzo A, Dice JF. (2003) Cathepsin A regulates chaperone-mediated autophagy through cleavage of the lysosomal receptor. EMBO JJan 2;22(1):47-59. de Geest, N.; Bonten, E.; Mann, L.; de Sousa-Hitzler, J.; Hahn, C.; d'Azzo, A. (2002)  Systemic and neurologic abnormalities distinguish the lysosomal disorders sialidosis and galactosialidosis in mice. Hum. Molec. Genet. 11: 1455-1464. Bonten EJ, d'Azzo A.(2000) Lysosomal neuraminidase. Catalytic activation in insect cells is controlled by the protective protein/cathepsin A. J Biol Chem. Dec 1;275(48):37657-63.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
100-401-396

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
200-4136

Product Type
GFP and Epitopes;
Applications
WB, IF
Catalog Number
200-4236

Product Type
GFP and Epitopes;
Applications
FLISA, WB, IF
Catalog Number
200-430-036

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry