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Primary Antibodies  >  AKT Signaling Antibodies
 

AKT Antibody

Rabbit Polyclonal
Code

100-401-401
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody.  
Tissue:  neonatal rat cardiomyocytes.  
Fixation:  0.5% PFA. 
Antigen retrieval:  not required.
Primary antibody:  AKT antibody at 1:80 dilution  for 1 h at RT.
Secondary antibody:  Texas-red™ conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  AKT is nuclear.
Staining:  Anti-AKT staining appears green.  Actin filaments are labeled red using a Texas-red™ conjugated phalloidin.
Western Blot of Rabbit Anti-AKT antibody.  
Lane 1:  NIH/3T3 whole cell lysate.  
Load:  20 ug per lane.
Primary antibody:  AKT antibody at 1:500 for overnight at 4°C.
Secondary antibody:  HRP conjugated Gt-a-Rabbit IgG (611-103-122) at 1:10,000 preceded color development using Pierce Chemical's SuperSignal™ substrate.
Block:  MOPS buffer overnight at 4°C.
Predicted/Observed size:  56 kDa, 56 kDa for AKT. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

AKT Antibody Properties

Description
Anti-AKT (RABBIT) Antibody - 100-401-401
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, chicken
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
IF Microscopy : 1:100 - 1:1,000
FlowCytometry : User Optimized
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AKT1
Download CofA
Lot#  

AKT Antibody Description

Background
AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen
AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide.  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type
Peptide
Storage Condition
Store Anti-Akt antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight,  immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
AKT Antibody reacts with the AKT from human tissues.  Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses.  J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14.
Specific Reference
Matsuda N, Takano Y, Kageyama S-I, et al. Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Cardiovasc. Res. 2007 Oct 1;76(1):132-140. Takeuchi R, Ryo A, Komitsu N, et al. Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study. Arthritis Res Ther. 2008;10(4):R77
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-269

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry