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Primary Antibodies  >  Stem Cell Antibodies
 

Notch 1 Antibody

Rabbit Polyclonal
Code

100-401-405
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Rockland's Anti-NOTCH 1 antibody was diluted 1:500 to detect NOTCH 1 in human brain cerebellum tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Synonyms: hN1 antibody, Neurogenic locus Notch homolog protein 1 antibody, Notch 1 intracellular domain antibody, Notch homolog 1 translocation associated antibody, TAN1 antibody, Translocation associated Notch protein TAN1 antibody

Notch 1 Antibody Properties

Description
Anti-NOTCH 1 (intra) (RABBIT) Antibody - 100-401-405
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
NOTCH1
Download CofA
Lot#  

Notch 1 Antibody Description

Background
Notch-1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Immunogen
Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2488-2502 of human Notch 1.  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Notch-1 Antibody shows reactivity at a 1:1000 dilution by western blot.  A strong response was detected by ELISA against the immunizing peptide. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Notch1 antibody is directed against human NOTCH 1.  No reaction is detected against NOTCH 2.  No reactivity was observed against Mouse Notch.  Other species have not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW. (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102. Gridley T.  (2003)  Notch signaling and inherited disease syndromes. Hum Mol Genet: 12: Suppl 1, R9-R13.
Specific Reference
Spyros Stylianou, Clarke RB, and Brennan K (2006) Aberrant Activation of Notch Signaling in Human Breast Cancer. Cancer Res February 1, 2006 66; 1517 doi: 10.1158/0008-5472.CAN-05-3054
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Product Type
Proteins and Peptides;
Catalog Number
000-000-405

Product Type
Proteins and Peptides;
Catalog Number
000-000-407

Product Type
Proteins and Peptides;
Catalog Number
000-000-408

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry