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Primary Antibodies  >  Stem Cell Antibodies
 

NOTCH 2 Antibody

Rabbit Polyclonal
Code

100-401-408
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Rockland's anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Synonyms: AGS2 antibody, hN2 antibody, Notch homolog 2 antibody, Notch2 antibody

NOTCH 2 Antibody Properties

Description
Anti-NOTCH 2 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-408
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:400 - 1:2,000
Immunochemistry: 1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
NOTCH2
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Lot#  

NOTCH 2 Antibody Description

Background
Anti-Notch 2 Antibody recognizes Notch 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Notch2 has a strong response was detected by ELISA against the immunizing peptide.This product was assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG [H&L] (Goat) (code #611-1302) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (code # ABTS-100) as a substrate for 30 minutes at room temperature.  A working dilution of 1:30,000 to 1:90,000 is suggested for this product.
Purity/Specifity
This antiserum is directed against human NOTCH 2.  Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2.  Only one amino acid difference (conservative) occurs in mouse and rat.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102. Gridley T  (2003)  Notch signaling and inherited disease syndromes. Hum. Mol. Genet.: 12,  Suppl 1, R9-R13.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-405

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
100-401-407

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
Human, mouse
Catalog Number
200-401-698

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry