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Primary Antibodies  >  Transcription Factor Antibodies
 

STAT4 Antibody

Rabbit Polyclonal
Code

100-401-861
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western blot following immunoprecipitation of STAT4 using ROCKLAND Immunochemical's Rabbit-anti-STAT4 antibody.  Lane 2 shows the detection of a single band corresponding to mouse STAT4.  Molecular weight markers (not shown) confirm a MW of ~ 85 kDa.  For IP use approximately 5 µl of antiserum.  A 1:5,000 dilution of the antiserum was used for western blotting.  Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) with visualization via ECL.  Film exposure approximately 45''.  Other detection systems will yield similar results.
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Synonyms: Signal transducer and activator of transcription 4 Stat4

STAT4 Antibody Properties

Description
Anti-STAT4 (RABBIT) Antibody - 100-401-861
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:5,000
ImmunoPrecipitation: Use 5 µL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
STAT4
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Lot#  

STAT4 Antibody Description

Background
Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription.  STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members.  STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 730-749 of Mouse STAT -4. Swiss Prot accession number P42228.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  A HeLa whole cell lysate or mouse testis lysate are suitable for use as a positive control. This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 µL for immunoprecipitation.
Purity/Specifity
This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.  Reactivity occurs against mouse STAT4 protein.  Cross reactivity with closely related STAT family members has not been determined.  Cross reactivity is expected against human STAT4 protein based on a 95% homology of the amino acid sequence of the immunogen between human and mouse.  A single amino acid change is reported (from N to K).   Cross reactivity is expected against rat STAT4.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Zhong,Z., et al. (1994) Stat3 and Stat4: members of the family of signal transducers and activators of transcription. Proc. Natl. Acad. Sci. U.S.A. 91 (11), 4806-4810. Yamamoto,K., et al. (1994) Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation. Mol. Cell. Biol. 14 (7), 4342-4349. Matikainen, S., et al. (1999) IFN-a activates multiple STAT proteins and upregulates proliferation associated IL-2Ra, c-myc, and pim-1 genes in human T cells. Blood 93: 1980-1991.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-A45

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-A45S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-B18

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse
Catalog Number
200-301-B65

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry