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PI3-kinase p110 delta Antibody

Rabbit Polyclonal
NCI Collaboration
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Immunoprecipitation and western blot using ROCKLAND Immunochemical's Rabbit-anti-PI3K p110d antibody.  Lane 1 shows the detection of a single band corresponding to mouse p110d detected in a lysate from p110d +/+ mice (lane 1) and the absence of staining in a similar lysate isolated from p110d -/- mice (lane 2).  Molecular weight markers confirm a MW of ~120 kDa. In both instances 2 mg of a total splenic lysate was used for immunoprecipitation and western blot analysis.  For IP use ~5 µl of antiserum.  For WB use a 1:5,000 dilution of antiserum.  Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) with visualization via ECL.  Film exposure approximately 45''.  Other detection systems will yield similar results.  See Jou et al for additional details.
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Number:
100-401-862
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

PI3-kinase p110 delta Antibody Properties

Description
Anti-PI3-kinase p110 d (delta subunit) (RABBIT) Antibody - 100-401-862
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, human
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:20,000
Western Blot : 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PIK3CD
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PI3-kinase p110 delta Antibody Description

Background
Phosphoinositide 3-kinases (PI3Ks) generate 3-phosphoinositide lipids in cell membranes.  A variety of intracellular target proteins interact with these lipids via specific lipid-binding modules and, as a consequence, undergo changes in their localization and/or activity. In this way, PI3Ks participate in the regulation of mitogenesis, differentiation, survival, intracellular vesicular transport, cytoskeletal reorganization, and motility.  Tyrosine kinases and Ras use PI3Ks as essential intracellular signal relay molecules. PI3Ks are heterodimeric enzymes consisting of a regulatory subunit in complex with a p110 catalytic subunit. Mammmals have genes encoding three distinct catalytic subunits (p110a, p110ß, and p110d and three regulatory subunits (p85a, p85ß, and p55d). All of the p110 isoforms are capable of interacting with each type of regulatory subunit. They are also similarly recruited to phosphotyrosine complexes and have, at least in vitro, the same lipid substrate specificity. However, it is becoming increasingly clear that PI3K isoforms differ in their interaction with Ras and regulation of lipid kinase activity, and in their protein kinase activities. Several groups have provided evidence that p110 isoforms have nonredundant functions in the regulation of cell proliferation, survival, actin cytoskeleton reorganization, and migration downstream of given receptors. This antibody is specific for the carboxy teminal end of p110d that is expressed predominantly in leukocytes.
Synonyms
p110D antibody, p110delta antibody, Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody, PI3K antibody, PIK3CD antibody
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of mouse PI3K p110d. This sequence is identical in both mouse and human.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  A mouse whole cell splenic lysate is suitable for use as a positive control.
Purity/Specifity
This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.  Reactivity occurs against mouse PI3K p110d subunit. Cross reactivity is expected against the human protein as the sequence of the immunogen is 100% identical in both human and mouse. Cross reactivity is also expected against rat PI3K p110d.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Puri,K.D. et al. (2004) Mechanisms and implications of phosphoinositide 3-kinase delta in promoting neutrophil trafficking into inflamed tissue. Blood 103 (9), 3448-3456. Sadhu,C. et al. (2003) Selective role of PI3K delta in neutrophil inflammatory responses. Biochem. Biophys. Res. Commun. 308 (4), 764-769. Clayton,E. et al. (2002) A crucial role for the p110delta subunit of phosphatidylinositol 3-kinase in B cell development and activation. J. Exp. Med. 196 (6), 753-763.
Specific Reference
Jou,S.T. et al. (2002) Essential, nonredundant role for the phosphoinositide 3-kinase p110delta in signaling by the B-cell receptor complex. Mol. Cell. Biol. 22 (24), 8580-8591. PUBMED 12446777
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human. mouse, rat, monkey
Catalog Number
200-301-269S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry