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Primary Antibodies  >  Stem Cell Antibodies

FBXO4 Antibody

Rabbit Polyclonal


100 µL


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Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose.  FBX4 was detected using Rockland’s anti-FBX4 at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
Immunocytochemistry staining of NIH-3T3 cells with Rockland’s anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia,
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$20.00 to United States
Synonyms: F box protein 4 antibody, F box protein Fbx4 antibody, FBX4 antibody, FBXO 4 antibody, FLJ10141 antibody

FBXO4 Antibody Properties

Anti-F-box only protein 4 (FBX4) [RABBIT) Antibody - 100-401-963
Target Species
Known Cross Reactivity
human, mouse
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 – 1:3000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
85 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
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FBXO4 Antibody Description

Skp/Cullin/F-box (SCF) complexes are part of a large family of E3 ubiquitin ligases in which F-box proteins determine substrate specificity.  Substrate selection by F-box proteins may also be aided by accessory cofactors.  The F-box only protein 4, FBX4, interacts via its F-box with SKP1 and CUL1 to form SCFFBX4 ubiquitin ligase.  The activity of the SCFFBX4 complex can be regulated by aB crystallin, a small heat shock protein which serves as a specificity cofactor for FBX4 dependent ubiquitination.  FBX4 together with aB crystallin (SCFFBX4-aB crystallin) promotes the ubiquitination of cyclin D1 phosphorylated at Thr-286.  In addition, the    SCFFBX4-aB crystallin ubiquitin ligase has been implicated in the ubiquitination of unknown substrates in desmin-related myopathy.  SCFFBX4, in the absence of aB crystallin, also ubiquitinates the telomeric protein PIN2/TRF1.
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product).
Immunogen Type
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum has been tested for use in western blotting and immunocytochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 44 kDa in size by western blotting in the appropriate cell lysate or extract.
This antiserum is directed against human and mouse FBX4. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lin DI, Barbash O, Kumar KGS, Weber JD, Harper JW, Klein-Szanto AJP, Rustgi A, Fuchs SY, Diehl JA (2006) Phosphorylation-dependent ubiquitination of cyclin D1 by the SCFFBX4-aB crystallin complex. Molecular Cell. In press. Lee TH, Perrem K, Harper JW, Lu KP, Zhou XZ (2006) The F-box protein FBX4 targets PIN2/TRF1 for ubiquitin-mediated degradation and regulates telomere maintenance. J. Biol. Chem. 281(2): 759-768. den Engelsman J, Keijsers V, de Jong WW, Boelens WC (2003) The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination. J. Biol. Chem. 278(7): 4699-4704. Chiaur DS, Murthy S, Cenciarelli C, Parks W, Loda M, Inghirami G, Demetrick D, Pagano M  (2000) Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors. Cytogenet. Cell Genet. 88(3-4): 255-258.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number

Product Type
Proteins and Peptides;
Catalog Number

Product Type
Primary Antibodies;
Reacts With
human, rat and mouse
Catalog Number

Product Type
Primary Antibodies;
Reacts With
human, mouse
Catalog Number

Product Label


Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry