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Primary Antibodies  >  Infectious Disease Antibodies
 

Sars-Cov Nonstructural Protein 8 Antibody

Rabbit Polyclonal
Code

100-401-A53
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Immunoprecipitation followed by western blotting using Rockland's Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A).  Immunofluorescence Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (611-104-122) was used.  Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
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Synonyms: Replicase polyprotein 1a, ORF1a polyprotein, nsp3, PL2-PRO, Papain-like proteinase, PL-PRO antibody, SARS coronavirus main proteinase, nsp4, 3CL-PRO, nsp5, nsp6, nsp7, nsp8, nsp9, nsp10, GFL, nsp11

Sars-Cov Nonstructural Protein 8 Antibody Properties

Description
Anti-SARS-CoV Nonstructural Protein 8 (nsp8) (RABBIT) Antibody - 100-401-A53
Host
Rabbit
Target Species
SARS
Known Cross Reactivity
SARS-Coronavirus nsp8 protein
Clonality
Polyclonal
Application
IF Microscopy : 1:300
Western Blot : 1:1,000
ImmunoPrecipitation: 1:60
Other Dilution: IMMUNOELECTRON MICROSCOPY 1:100
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
NSP8
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Sars-Cov Nonstructural Protein 8 Antibody Description

Background
The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes.  No specific information on the function of nsp8 is available.
Immunogen
This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA.  After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
Purity/Specifity
This antibody is directed against SARS-Coronavirus nsp8 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Snijder, E. J., P. J. Bredenbeek, J. C. Dobbe, V. Thiel, J. Ziebuhr, L. L. M. Poon, Y. Guan, M. Rozanov, W. J. M. Spaan, and A. E. Gorbalenya. 2003. Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J. Mol. Bio. 331:991-1004. Prentice, E., J. McAuliffe, X. T. Lu, K. Subbarao, and M. R. Denison. 2004. Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins. J. Virol. 78:9977-9986. Snijder, E.J., van der Meer, Y., Zevenhoven-Dobbe, J.C., Onderwater, J.J.M., van der Meulen, J., Koerten, H.K., and Mommaas, A.M. 2006. Ultrastructure and origin of membrane vesicles associated with the SARS-coronavirus replication complex. Manuscript in preparation.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
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Catalog Number
009-001-310

Product Type
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Reacts With
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Catalog Number
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Product Type
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Reacts With
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Catalog Number
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Product Type
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Reacts With
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Catalog Number
009-001-B95

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry