House Secondary Banner
 
You may also want to consider these related products.
Anti-ALPHA AMYLASE (Bacillus amyloliquefaciens) (RABBIT) Antibody - 100-4145
Anti-ALPHA AMYLASE (Bacillus amyloliquefaciens) (RABBIT) Antibody - 100-4145
Anti-DEOXYRIBONUCLEASE I (Bovine Pancreas) (RABBIT) Antibody Biotin Conjugated - 200-4661
Anti-DEOXYRIBONUCLEASE I (Bovine Pancreas) (RABBIT) Antibody Biotin Conjugated - 200-4661
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Primary Antibodies  >  Sample Size Primary Antibodies
 

Liver Carboxylesterase-1 Antibody

Rabbit Polyclonal
Code

100-401-B14S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western blot using Rockland's anti-hCE1 antibody shows detection of hCE1 (arrowhead) transfected into U373 MG (glioblastoma) whole cell lysate (lane 2) as a 66 kDa band.  Purified hCE1 (lane 4) is similarly detected.  The higher molecular weight band in lane 4 may represent a trimeric form of hCE1.  No reactivity is seen in the untransfected control (lane 1).  No cross reactivity occurs against the human intestinal form of CES1 (lane 3). The primary antibody was used at a 1:2,500 to detect purified hCE1. Personal communication, Philip Potter and Jason Hatfield, St. Jude Children’s Research Hospital, Memphis, TN.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Liver carboxylesterase 1, EC=3.1.1.1 Acyl coenzyme A:cholesterol acyltransferase ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH,

Liver Carboxylesterase-1 Antibody Properties

Description
Anti-Human Liver Carboxylesterase-1 (RABBIT) Antibody - 100-401-B14S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:2,500 – 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CES1
Download CofA
Lot#  

Liver Carboxylesterase-1 Antibody Description

Background
Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase).  Specific conditions for reactivity should be optimized by the end user.   Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract.  In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.   The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie. IL-1 stimulated hCE1 production from fibroblasts).
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bushman F. (2003) Targeting Survival Integration Site Selection by Retroviruses and LTR-Retrotransposons. Cell 115: 135-138. Danks MK, Morton CL, Krull EJ, Cheshire PJ, Richmond LB, Naeve CW, Pawlik CA, Houghton PJ, and Potter PM. Comparison of Activation of CPT-11 by Rabbit and Human Carboxylesterases for Use in Enzyme/Prodrug Therapy Clin. Cancer Res., 5:917-924, 1999. Bencharit S, Morton CL, Xue Y, Potter PM, Redinbo MR. (2003) Structural basis of heroin and cocaine metabolism by a promiscuous human drug-processing enzyme. Nat Struct Biol 10:349-356
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
Bacillus amyloliquefaciens
Catalog Number
100-4145


Product Type

Applications
ELISA, WB
Reacts With
Bos taurus (Bovine)
Catalog Number
200-4661

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry