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Scythe/Bat3 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot showing detection of mouse Scythe/BAT3 by Rockland's anti-Mouse Scythe/BAT3 antibody in various tissues of mouse WT and Scythe knockout (-/-) embryos.
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Number:
100-401-B63S
Size:
25 µL
Price:
$95.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Scythe/Bat3 Antibody Properties

Description
Anti-Mouse Scythe/BAT3 (RABBIT) Antibody - 100-401-B63S
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human, rat, mouse
Clonality
Polyclonal
Application
ELISA : User Optimized
Western Blot : 1:5,000 to 1:8,000
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BAT3
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Scythe/Bat3 Antibody Description

Background
Scythe protein (also known as Bat3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. Scythe binds Reaper, a potent apoptotic inducer. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. Scythe interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. Scythe and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. HLA-B-associated transcript 3 (Bat3/Scythe) is essential for p300-mediated acetylation of p53. Bat3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.
Synonyms
Bat3, HLA-B-associated transcript 3, Large proline-rich protein BAT3
Immunogen
This Bat3 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/Bat3.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This Scythe polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200kDa in size corresponding to Scythe/Bat3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/Bat3 may appear corresponding to variable amount of splice isoforms present.
Purity/Specifity
This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01%. The antibody detects endogenous Scythe/Bat3 in cell lysates.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Fabienne Desmots, Helen R. Russell, Youngsoo Lee, Kelli Boyd, and Peter J. McKinnon (2005). The Reaper-Binding Protein Scythe Modulates Apoptosis and Proliferation during Mammalian Development. Molecular and Cellular Biology, December 2005, p. 10329-10337, Vol. 25, No. 23.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, rat
Catalog Number
600-401-412

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry