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Glucose Oxidase Antibody

Rabbit Polyclonal
NCI Collaboration
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Number:
100-4137
Size:
2 mL
Price:
$309.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Glucose Oxidase Antibody Properties

Description
Anti-GLUCOSE OXIDASE (RABBIT) Antibody - 100-4137
Host
Rabbit
Target Species
Aspergillus niger
Known Cross Reactivity
Aspergillus niger
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:3,000
Physical State
Lyophilized
Shipping Condition
Ambient
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
gox
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Glucose Oxidase Antibody Description

Background
Glucose Oxidase, first described in Aspergillus niger), catalyzes the oxidation of glucose into gluconic acid whilst in an aerobic environment. Glucose Oxidase is one of the most widely used biomarkers due to the large enthalpy change observed when it oxidizes glucose. Glucose oxidase can be used as a label in immunoassays when attached to antibodies and for staining tissues. In respect to glucose oxidases' ability to stain tissues, it is most commonly used to determine the levels of glucose in blood, serum, and plasma, as part of colorimetric assays. Anti-Glucose Oxidase Antibody is ideal for investigators in Immunology, Molecular Biology, and Hematology Research.
Synonyms
Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
Immunogen
Glucose Oxidase [Aspergillus niger]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Glucose Oxidase Antibody has been assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Rabbit) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for Anti-Glucose Oxidase Antibody.
Purity/Specifity
Anti-Glucose Oxidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Oxidase [Aspergillus niger].  Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Wilson, R. and A. P. F. Turner. (1992). Glucose Oxidase: An Ideal Enzyme. Biosensors and Bioelectronics. Vol. (7): 165-185. Julio Raba and Horacio A. Mottola. (1995). "Glucose Oxidase as an Analytical Reagent". Critical Reviews in Analytical Chemistry 25 (1): 1–42.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Aspergillus niger
Catalog Number
100-1137

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Aspergillus niger
Catalog Number
100-6137

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Aspergillus niger
Catalog Number
200-101-037

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry