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Primary Antibodies  >  Enzyme Antibodies
 

Alpha Amylase Antibody

Rabbit Polyclonal
Code

100-4145
Size

2 mL
Price

$309.00
Availability

Ships next business day.
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Synonyms: Alpha-amylase EC=3.2.1.1 1,4-alpha-D-glucan glucanohydrolase

Alpha Amylase Antibody Properties

Description
Anti-ALPHA AMYLASE (Bacillus amyloliquefaciens) (RABBIT) Antibody - 100-4145
Host
Rabbit
Target Species
Bacillus amyloliquefaciens
Known Cross Reactivity
Bacillus amyloliquefaciens
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Alpha-amylase
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Alpha Amylase Antibody Description

Background
Alpha Amylase is an enzyme that begins the digestion of starches. Specifically, Alpha Amylase cleaves the alpha bonds in large polysaccharides. Alpha Amylase is found in both the pancreas and saliva in humans, with salivary amylase beginning the digestion of starches and pancreatic amylase finishing the digestion. The large amount of conserved amino acid sequences and prevalence of alpha amylase enzymes has allowed this class of enzymes to be beneficial to industrial breakdown of starches into glucose and high-fructose corn syrup. Alpha amylase derived from bacillus amyloliquefaciens is also useful in the production of various detergents relying on the breakdown of starches. Anti-Alpha Amylase (Bacillus amyloliquefaciens) Antibody is ideal for investigators in Enzymology, Molecular Biology, and Microbiology research.
Immunogen
a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Purity/Specifity
Anti-Alpha Amylase (Bacillus amyloliquefaciens) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified a-Amylase [Bacillus amyloliquefaciens].  Cross reactivity against a-Amylase from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Goodsell, David. "Alpha-Amylase." RCSB Protein Data Bank. Feb. 2006.
Specific Reference
van der Maarel, van der Veen, Uitdehaag, Leemhuis, and Dijkhuizen. "Properties and applications of starch-converting enzymes of the alpha-amylase family." J Biotechnol. 2002 Mar 28; 94(2): 137-55. "The use of enzymes in detergents." Science and the Built Environment, London South Bank University. 20 December 2004.
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bacillus amyloliquefaciens
Catalog Number
200-4145

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Bacillus amyloliquefaciens
Catalog Number
200-4345


Product Type

Applications
ELISA, WB
Reacts With
Bacillus amyloliquefaciens
Catalog Number
200-4645

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry