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NFkB p65 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit anti-NFKB p65 N term antibody.  
Lane 1-4:  PC3 cell lysate.   
Load:  20, 30, 40, and 50 ug per lane.
Primary antibody:  NFKB p65  antibody at 1:5000 for overnight at 4°C.
Secondary antibody:  HRP rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~65kDa for NFKB p65. 
Other band(s): ~110kDa and ~45kDa.
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Number:
100-4165N
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

NFkB p65 Antibody Properties

Description
Anti-NFKB p65 (Rel A) N-TERMINAL SPECIFIC (RABBIT) Antibody - 100-4165N
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:5000 - 1:10,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
82 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
NFKB1
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NFkB p65 Antibody Description

Background
Anti-NFkB p65 Antibody detects NfkB p65. NFkB-p65 a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Anti-NFkB p65 Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.
Synonyms
NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Immunogen
Anti-NFkB p65 Antibody was produced by repeated immunizations with a synthetic NFkB p65 peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-NFkB p65 (N-terminal specific) is suitable for the detection by immunoblot of human NFkB p65 (N-terminal specific).  Functionality in supershift assays has not been determined.
Purity/Specifity
NFkB p65 Antibody was prepared from monospecific antiserum by delipidation and defibrination.  Anti-NFkB p65 (N-terminal specific) may react non-specifically with other proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-264

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-4164

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, GS, Other
Reacts With
human, mouse, rat
Catalog Number
100-4165

Product Type
Primary Antibodies;
Applications
ELISA, WB, GS, Other
Reacts With
human
Catalog Number
100-4166

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry