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Primary Antibodies  >  Transcription Factor Antibodies
 

IKB alpha Antibody

Rabbit Polyclonal
Code

100-4167C
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Synonyms: IkappaBalpha antibody, IKBA antibody, IKBalpha antibody, MAD3 antibody, NF kappa B inhibitor alpha antibody, NFKBI antibody, NFKBIA antibody

IKB alpha Antibody Properties

Description
Anti-IKBa C-terminal (RABBIT) Antibody - 100-4167C
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
IKBa
Download CofA
Lot#  

IKB alpha Antibody Description

Background
Anti-IKB alpha Antibody detects IkBa. I-kappa-B-alpha inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, IKBA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. Anti-IKBa Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Immunology research.
Immunogen
IKB alpha Antibody was produced by repeated immunizations with a synthetic IkBa peptide corresponding to a region near the C-terminus of the human protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-IkBa Antibody is suitable for western blotting, ELISA, and IHC. Researchers should determine optimal titers for applications that are not stated below.
Purity/Specifity
IKB alpha Antibody was prepared from monospecific antiserum by delipidation and defibrination.  Anti- IkBa may react non-specifically with other proteins.   Control peptide (code #100-4167P) will compete only with the specific reaction of antiserum with IkBa.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Hoffmann, A. et al. (2002) The IkB-NFkB Signaling Module: Temporal Control and Selective Gene Activation. Science 298:8; pp 1241-1245.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-401-219

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
100-401-220

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-4186

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry