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Primary Antibodies  >  Stem Cell Antibodies
 

TNF p55 Receptor Antibody

Rabbit Polyclonal
Code

109-401-308
Size

1 mL
Price

$355.00
Availability

Ships next business day.
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Synonyms: TBP1 antibody, TNFR1 antibody, TNFR55 antibody, TNFRSF1A antibody, Tumor necrosis factor alpha receptor antibody

TNF p55 Receptor Antibody Properties

Description
Anti-Human TNF p55 Receptor (sTNFRp55) (RABBIT) Antibody - 109-401-308
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, primate
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
None
Stabilizer
None
GeneName
TNFR-I
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Lot#  

TNF p55 Receptor Antibody Description

Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum against Human TNF p55 Receptor (sTNFRp55) has been tested for use in ELISA,  radioimmunoassays, immunoblotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot  using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application.  This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2,000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody  in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay. 
Purity/Specifity
This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against the extracellular domain of the cell bound human TNFRp55.  In general, this antibody also detects primate sTNFRp55.  The antibody does not recognize human sTNFRp75.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-306

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-401-219

Product Type
Proteins and Peptides;
Catalog Number
100-401-219p

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, primate
Catalog Number
109-401-306

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry