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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-6 Antibody

Rabbit Polyclonal
Code

109-401-310
Size

1 mL
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody.  Molecular weight markers are also shown (MW).  After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST.   Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate.  Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF

IL-6 Antibody Properties

Description
Anti-Human IL-6 (RABBIT) Antibody - 109-401-310
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:400 - 1:800
ImmunoPrecipitation: 1:400 - 1:800
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
73 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
None
GeneName
IL6
Download CofA
Lot#  

IL-6 Antibody Description

Background
Anti IL-6 Antibody recognizes IL-6 that is a secreted cytokine with a wide variety of biological functions. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Immunology and Cancer research
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
IL-6 antisera has been tested for use in neutralizations, ELISA, immuno-histochemistry, radioimmunoassay, immunoprecipitation, and immunoblotting.  Reactivity in other immunoassays is unknown. For immunoblotting use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).  PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS.  For immunohistochemistry either paraffin fix or cryofix tissue.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.
Purity/Specifity
Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  The antiserum is also useful for neutralization of human of IL-6 activity in bioassays.  For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.  In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6.  However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Novick, D., et al. (1989) Monoclonal antibodies for affinity Purification of IL-6/IFNveta 2 and for neutralization of HGF activity. Hybridoma 8: 561-567. Novick, D., et al. (1992) Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody. Cytokine 4 (1): 6-11. Silvera, M.R., et al. (1994) Expression of TGFa isoforms by thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGFa as a regulator of IL-dependent stimulation of IL-6. Lymphokine Cytokine Res 13: 277-285.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-310

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
Human
Catalog Number
109-401-311

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry