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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

IL-10 Antibody

Rabbit Polyclonal
Code

109-401-312
Size

1 mL
Price

$309.00
Availability

Ships next business day.
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Rockland Anti IL-10 whole antiserum (109-401-312) was tested against recombinant IL10.  Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose.  Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n 109-401-312 diluted 1:200 in 3% BSA-TBS o/n 4oC.  Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate.  Blot was imaged with VersaDoc Imaging system by Biorad.  Other detection systems will yield similar results.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, Interleukin 10 antibody, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody

IL-10 Antibody Properties

Description
Anti-IL-10 (RABBIT) Antibody - 109-401-312
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IL-10
Download CofA
Lot#  

IL-10 Antibody Description

Background
Anti IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-?B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.
Immunogen
The whole rabbit serum was prepared by repeated immunizations with human IL-10.
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting.  The antibody recognizes hrIL-10.  Reactivity in other immunoassays is unknown.
Purity/Specifity
The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts).   In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.  The antiserum is also useful for neutralization of human of IL-10 activity in bioassays.  For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.  The antibody will not neutralize the biological activity of murine IL-10.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-301-B31

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
209-301-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB, FC
Reacts With
human
Catalog Number
209-301-C58

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B31

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry