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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

GM-CSF Antibody

Rabbit Polyclonal
NCI Collaboration
Order Offline
Number:
109-401-314
Size:
1 mL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

GM-CSF Antibody Properties

Description
Anti-GM-CSF (RABBIT) Antibody - 109-401-314
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
None
Stabilizer
None
GeneName
CSF2
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GM-CSF Antibody Description

Background
Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor; CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
Synonyms
Molgramostin antibody, Pluripoietin Alpha antibody, Sargramostim antibody, Colony stimulating factor antibody, CSF 2 antibody, CSF Alpha antibody, Burst Promoting Activity antibody
Immunogen
This whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum against Human GM-CSF has been tested for use in neutralizations, ELISA and immunoblotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method.
Purity/Specifity
This antiserum has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-A45

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-B92

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-406-B92

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry