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Secondary Antibodies  >  IgG Fraction Antibodies
 

Mouse IgG (H&L) Antibody

Goat Polyclonal
Code

110-1102
Size

2 mL
Price

$105.00
Availability

Ships next business day.
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Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule. Lane 2: Mouse IgG F(c) Fragment. Lane 3: Mouse IgG F(ab) Fragment. Lane 4: Mouse IgM Kappa. Lane 5: Mouse Serum. Load: 50ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n 805-703-125)1:40,000 for 30 min at RT .  Predicted/Obsevered Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.
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Shipping:
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Handling:
$20.00
Synonyms: Goat Anti-Mouse Ab, Mouse Secondary Antibody, Mouse Antibody in Goat, Gt-a-Mouse Antibody

Mouse IgG (H&L) Antibody Properties

Description
Anti-MOUSE IgG (H&L) (GOAT) Antibody - 110-1102
Host
Goat
Specificity
IgG (H&L)
Target Species
Mouse
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

Mouse IgG (H&L) Antibody Description

Background
MOUSE IgG (H&L) (GOAT) Antibody generated in sheep detects specifically mouse IgG whole molecule. Anti-mouse IgG antibody is ideal for investigators involved in serum protein component research.
Immunogen
Mouse IgG whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Anti-MOUSE IgG (H&L) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific condtions should be optimized by user.
Purity/Specifity
Anti-MOUSE IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.  Anti-MOUSE IgG (H&L) (GOAT) Antibody Assay by immunoelectrophoresis resulted in a single precipitin arc against Mouse IgG and Mouse Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Type
Kits;
Applications
WB
Reacts With
Anti-Mouse IgG
Catalog Number
KCA002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry