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NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
Primary Antibodies  >  Glucose Energy Metabolism Antibodies
 

Glucose Oxidase Antibody

Goat Polyclonal
Code

200-101-037
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Rockland Goat anti Glucose Oxidase antibody was used to detect Glucose Oxidase under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of  ~1 and 0.25 ug of protein per lane were run by SDS-PAGE.  Protein was transferred to nitrocellulose and probed with Rockland Goat anti Glucose Oxidase antibody (200-1137 lot 165 1:5K in MB-0070, ON 4 C).  Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 lot 20834 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system
Western Blot of Goat anti-Glucose Oxidase antibody.  
Lane 1:  Glucose Oxidase.  
Lane 2:  None.  
Load:  50 ng per lane.
Primary antibody:  Glucose Oxidase antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  65-70 kDa, 65-70 kDa for Glucose Oxidase. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Glucose Oxidase Antibody Properties

Description
Anti-GLUCOSE OXIDASE (GOAT) Antibody - 200-101-037
Host
Goat
Target Species
Aspergillus niger
Known Cross Reactivity
Aspergillus niger
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:1000 - 1:5000
Immunohistochemistry: User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
gox
Download CofA
Lot#  

Glucose Oxidase Antibody Description

Immunogen
Glucose Oxidase [Aspergillus niger]
Immunogen Type
Native Protein
Storage Condition
Store Glucose Oxidase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Purity/Specifity
This Anti-Glucose Oxidase Antibody product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].  Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
FLISA, WB
Catalog Number
605-431-013

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Catalog Number
605-441-013

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Catalog Number
605-451-013

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry