Anti-NFKB p65 (Rel A) Antibody - Immunofluorescence Microscopy

Anti-NFKB p65 (Rel A) Antibody - Western Blot

Anti-NFKB p65 (Rel A) Antibody – Immunohistochemistry

Anti-NFKB p65 (Rel A) Antibody – Immunocytochemistry

PAGE-MAP (microsphere affinity proteomics) of Mouse Anti-NFKB p65 (Rel A) Antibody

Datasheet

NFkB p65 Antibody

Name: NFkB p65 Antibody
Brand: Rockland Immunochemicals
Price: 485.0 USD
Availability: InStock

Mouse Monoclonal 27F9.G4 IgG2a kappa

5 References

Item No. 200-301-065

Size: 100 µg

Applications: WB, IHC, IF, IP

Reactivity: Human

Host Species: Mouse

$485.00 /Per Item

25 µL $125.00

100 µg $485.00

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Background

NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research.

Expand Background

Specifications for NFkB p65 Antibody

Product Details

Description: Anti-NFKB p65 (Rel A) (MOUSE) Monoclonal Antibody - 200-301-065

Synonyms: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

Host Species: Mouse

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: RELA - View All RELA Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Purity/Specificity: Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody. Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.

Database Links:

UniProtKB - Q04206

NCBI - 223468676

GeneID - 5970

Application Details

Tested Applications: IF, IHC, WB

Suggested Applications: IP - View References

Application Note: Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.98 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (5)

Windmöller, BA et al. (2022). Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-derived Stem Cells. Stem Cell Reviews and Reports

Applications

IHC, ICC, Histology

PubMed

Chansard A et al. (2021). Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols. Front Immunol.

Applications

IF, Confocal Microscopy

PubMed

Sikorski et al. (2018). A high-throughput pipeline for validation of antibodies. Nature Methods

Applications

PAGE-MAP, IP-MS

PubMed

Ryu, S et al. (2015). Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice. PloS One

Applications

IF, Confocal Microscopy

PubMed

Merkhofer EC et al. (2010). Her2 Activates NF-κB and Induces Invasion Through the Canonical Pathway Involving IKKα. Oncogene.

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

Immunoprecipitation (IP) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.