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Datasheet

Cdc2 p34 Antibody

Mouse Monoclonal POH-1 IgG2a kappa

200-301-160
100 µg
WB
Human
Mouse
$485.00 /Per Item
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Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphorylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.

Product Details

Anti-CDC2 (p34) (MOUSE) Monoclonal Antibody - 200-301-160
mouse anti-Cdc2 antibody, mouse anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, CDC28A, CDKN1, P34CDC2
Mouse
Monoclonal
IgG2a

Target Details

CDK1  - View All CDK1 Products
Human
Recombinant Protein
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines.  This antibody is not cross reactive with other cyclin dependent kinases.  Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur.  This reagent has broad interspecies reactivity.

Application Details

WB
Anti-p34 cdc2 has been tested by western blot and is suitable for immunohistochemistry, immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control.   Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.

Formulation

Liquid (sterile filtered)
1.023 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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