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Primary Antibodies  >  Sample Size Primary Antibodies
 

Akt phospho T308 Antibody

Mouse Monoclonal 18F3.H11 IgG1 kappa
Code

200-301-269S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Immunohistochemistry of Mouse anti-AKT pT308 antibody.  
Tissue:  human brain cerebellum tissue (40X).    
Fixation:  formalin fixed paraffin embedded.  
Antigen retrieval:  not required.
Primary antibody:  AKT pT308antibody  at 20 µg/mL  for 1 h at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear.
Staining:  AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of Mouse anti-AKT pT308 antibody.  
Lane 1:  non-phosphorylated AKT in untreated cells .  
Lane 2:  phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates .  
Load:  15 µg per lane.
Primary antibody:  AKT pT308 antibody at a 1:4,000 dilution in TBS with 3% BSA, for 3 h at 4° C.
Secondary antibody:   peroxidase conjugated Gt-a-Mouse IgG (Fc) (p/n 610-1303) was used at a 1:40,000 dilution for 1 h at 4° C.
Block:   3% BSA (p/n BSA-30) in TBS for 30 min at RT.
Predicted/Observed size:  (indicated by arrowhead at ~56 kDa). 
Other band(s): unspecific.
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Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

Akt phospho T308 Antibody Properties

Description
Anti-AKT pT308 (MOUSE) Monoclonal Antibody - 200-301-269S
Host
Mouse
Target Species
Human
Known Cross Reactivity
human. mouse, rat, monkey
Clonality
Monoclonal 18F3.H11 IgG1 kappa
Application
ELISA : 1:20,000
FlowCytometry : User Optimized
Western Blot : 1:500 - 1:3,000
Immunochemistry: 20 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AKT1
Download CofA
Lot#  

Akt phospho T308 Antibody Description

Background
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen
Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Anti-AKT pT308 antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting.  Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.  Specific conditions for reactivity should be optimized by the end user.  Use formalin-fixed paraffin-embedded sections for immunohistochemistry.    No pre-treatment of sample is required.
Purity/Specifity
Anti-AKT Phospho pT308 antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308.  A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence.  Cross-reactivity with AKT from other sources has not been determined.  Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991) Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, chimpanzee
Catalog Number
200-301-401

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry