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Anti-AKT (MOUSE) Monoclonal Antibody - 200-301-401
Primary Antibodies  >  AKT Signaling Antibodies
 

AKT Antibody

Mouse Monoclonal 14E5.16C8.25F6
Code

200-301-401
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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ELISA of Mouse Anti-AKT Antibody.  
Antigen: HCT-116 cell line.
Coating amount:  Confluent in the 96 well plate.  
Primary antibody:  AKT or GAPDH antibody at 2 µg/mL.  
Dilution series:  Primary and Secondary Antibodies 2-fold. 
Mid-point concentration:  N/A.
Secondary antibody:  DyLight™ 680 donkey secondary antibody and DyLight™ 800 goat secondary antibody starting at 1:1,000. 
Substrate:  None.
Western Blot of Mouse anti-AKT antibody.  
Lane 1:  unstimulated NIH/3T3 cell lysates.  
Lane 2:  PDGF stimulated NIH/3T3 cell lysates.  
Load:  10 µg per lane.
Primary antibody:  AKT antibody at 1:400 for overnight at 4°C.
Secondary antibody:  HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100).  
Block:   5% BLOTTO (p/n B501-0500 in TBS for 2h at RT.
Observed size: ~56 kDa for AKT. 
Other band(s): none.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

AKT Antibody Properties

Description
Anti-AKT (MOUSE) Monoclonal Antibody - 200-301-401
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse, rat, chimpanzee
Clonality
Monoclonal 14E5.16C8.25F6
Application
ELISA : 1:20,000
FlowCytometry : User Optimized
Western Blot : 1:500 - 1:3,000
Immunochemistry: 20 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AKT1, AKT2, AKT3
Download CofA
Lot#  

AKT Antibody Description

Background
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen
Anti-AKT Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues internal to the human AKT1 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-AKT Antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 54 - 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Purity/Specifity
Anti-AKT Antibody was purified by Protein A chromatography. This antibody is specific for human and mouse AKT protein.  A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence.  Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.   AKT antibody is suitable for Cell Signaling, Neuroscience, Signal Transduction research.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991) Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175. Staal,S.P. (1987) Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 84 (14), 5034-5037.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, chimpanzee
Catalog Number
200-301-401

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry