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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

BUBR1 Kinase Kinase Antibody

Mouse Monoclonal 8G1 IgG1
Code

200-301-902
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Synonyms: Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody

BUBR1 Kinase Kinase Antibody Properties

Description
Anti-BUBR1 Kinase (MOUSE) Monoclonal Antibody - 200-301-902
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal 8G1 IgG1
Application
ELISA : 1:5,000 - 1:20,000
IF Microscopy : 1:200 - 1:1,000
Western Blot : 1:200 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BUBR1
Download CofA
Lot#  

BUBR1 Kinase Kinase Antibody Description

Background
BUBR1 Kinase (also called Mitotic checkpoint serine/threonine-protein kinase, BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L and SSK1) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores.  This protein is highly expressed in thymus followed by spleen.  Defects in BUB1B are associated with tumor formation.
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.  The use of HeLa whole cell lysates is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8.  Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
Purity/Specifity
This Protein A purified antibody is directed against human BUBR1 Kinase protein. The product was purified from tissue culture supernatant by chromatography.  This antibody has only been tested on human cells.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF, IP
Reacts With
human
Catalog Number
200-301-901


Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry