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Primary Antibodies  >  Chromatin Antibodies
 

Lysine Acetylated Antibody

Mouse Monoclonal 7F8 IgG1
Code

200-301-A97
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Lysine Acetylated Antibody Properties

Description
Anti-Lysine Acetylated (AcK) (MOUSE) Monoclonal Antibody - 200-301-A97
Host
Mouse
Known Cross Reactivity
mammalian and avian
Clonality
Monoclonal 7F8 IgG1
Application
ELISA : User Optimized
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
See application note.
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
50% (v/v) Glycerol
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Lot#  

Lysine Acetylated Antibody Description

Background
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A common posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Ne-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).
Immunogen
This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanine (KLH).
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 1 µg of antibody is sufficient to detect 20 µg of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110). Antibody provided in PBS pH 7.4.
Purity/Specifity
This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues.  This antibody is known to react with acetylated lysine residues from mammalian and avian sources.  Reactivity with acetylated lysines from other sources is also expected.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Yang XJ. (2005). Oncogene. 24:1653-1662. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. Yang XJ. (2004). Bioessays 26:1076-1087. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP, FC
Reacts With
mammalian, avian
Catalog Number
600-401-939


Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry