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Primary Antibodies  >  Stem Cell Antibodies
 

LGR4 Antibody

Mouse Monoclonal 6G8.B3.G5.C3 IgG2b kappa
Code

200-301-B45
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Rockland's anti-LGR4 monoclonal antibody was used diluted to 5 ?g/ml to detect LGR4 staining at the membrane of cells in various human tissues.  A.  Brain cerebellum.  B. Pancreas islet.  Strongly positive staining is noted in subsets of cells within the islets of Langerhans.   Moderately positive staiing was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes.  Some elastin background staining is noted.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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Synonyms: LGR4 leucine-rich repeat-containing G protein-coupled receptor 4

LGR4 Antibody Properties

Description
Anti-LGR4 (MOUSE) Monoclonal Antibody - 200-301-B45
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, chimpanzee, macaque
Clonality
Monoclonal 6G8.B3.G5.C3 IgG2b kappa
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 5 µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG2b
Label
Concentration
1.13 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
LGR4
Download CofA
Lot#  

LGR4 Antibody Description

Background
LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
Purity/Specifity
This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human LGR4 protein. A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Weng,J., Luo,J., Cheng,X., Jin,C., Zhou,X., Qu,J., Tu,L., Ai,D.,Li,D., Wang,J., Martin,J.F., Amendt,B.A. and Liu,M.(2008) Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2. Proc. Natl. Acad. Sci. U.S.A. 105 (16), 6081-6086. Gao,Y., Kitagawa,K., Hiramatsu,Y., Kikuchi,H., Isobe,T., Shimada,M., Uchida,C., Hattori,T., Oda,T., Nakayama,K., Nakayama,K.I., Tanaka,T., Konno,H. and Kitagawa,M. (2006) Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. Cancer Res. 66 (24), 11623-11631. Loh,E.D., Broussard,S.R. and Kolakowski,L.F. (2001) Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor. Biochem. Biophys. Res. Commun. 282 (3), 757-764. Loh,E.D., Broussard,S.R., Liu,Q., Copeland,N.G., Gilbert,D.J., Jenkins,N.A. and Kolakowski,L.F. Jr. (2000) Chromosomal localization of GPR48, a novel glycoprotein hormone receptor like GPCR, in human and mouse with radiation hybrid and interspecific backcross mapping. Cytogenet. Cell Genet. 89 (1-2), 2-5. Hsu,S.Y., Liang,S.G. and Hsueh,A.J. (1998) Characterization of two LGR genes homologous to gonadotropin and thyrotropin receptors with extracellular leucine-rich repeats and a G protein-coupled, seven-transmembrane region. Mol. Endocrinol. 12 (12), 1830-1845.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
200-301-B45S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
200-301-B46

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Kits;
Applications
ELISA, WB, IHC, IF, IP
Catalog Number
K-500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry