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Datasheet

Anti-Peroxidase (Mouse) Antibody

Mouse Polyclonal

1 References
200-3138 200-3138S 200-3138-0100
50 mg 25 µL 100 µg
WB, ELISA, IF
Peroxidase
Mouse
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Background

Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Mouse) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.

Product Details

Anti-Peroxidase (Horseradish) (MOUSE) Antibody (BULK ORDER) - 200-3138
Mouse anti-peroxidase antibody, mouse anti-HRP, Horseradish peroxidase
Mouse
Polyclonal
IgG

Target Details

PRXC, HRPC
Peroxidase
Native Protein
Peroxidase [Horseradish]
Anti-Peroxidase (horseradish) (Mouse) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish].  Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Application Details

ELISA, WB
IF  - View References
Anti-Peroxidase (Horseradish) (Mouse) antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoprecipitation, conjugation, and most immunological methods requiring high titer and specificity.

Formulation

Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
None
None
5.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
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Applications
Proximity Ligation Assay (PLA)

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.