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Primary Antibodies  >  Enzyme Antibodies
 

Ovalbumin Antibody

Rabbit Polyclonal
Code

200-401-033
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Rabbit anti-Ovalbumin antibody.  
Lane 1:  ~1 µg Ovalbumin protein reduced.  
Lane 2:  0.25 µg Ovalbumin protein reduced.  
Lane 3:  ~1 µg Ovalbumin protein non-reduced.  
Lane 4:  0.25 µg Ovalbumin protein non-reduced. 
Primary antibody:  Ovalbumin antibody at 1:5000 for overnight at 4°C.
Secondary antibody:  Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hr at RT.
Block:  MB-070 buffer overnight at 4°C.
Predicted/Observed size:  42.9 kDa,  ~36 kDa for ovalbumin. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Ovalbumin Egg albumin Plakalbumin Allergen Gal d II Allergen=Gal d 2

Ovalbumin Antibody Properties

Description
Anti-OVALBUMIN (Hen Egg White) (RABBIT) Antibody - 200-401-033
Host
Rabbit
Target Species
Chicken
Known Cross Reactivity
Chicken (Gallus gallus)
Clonality
Polyclonal
Application
ELISA : 1:25,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:300 - 1:2,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SERPINB14
Download CofA
Lot#  

Ovalbumin Antibody Description

Background
Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.
Immunogen
Ovalbumin [Hen Egg White]
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].  Cross reactivity against Ovalbumin from other sources is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wang, J., Zhang, Y., Wei, J., Zhang, X., Zhang, B., Zhu, Z., Zou, W., Wang, Y., Mou, Z., Ni, B. and Wu, Y. (2007), Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response. Immunology, 121: 174–182. doi: 10.1111/j.1365-2567.2007.02554.x
Specific Reference
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Product Type

Applications
ELISA, WB, IHC
Reacts With
rabbit
Catalog Number
211-1301



Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry