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Primary Antibodies  >  Cancer Research Antibodies
 

Bcl-2 Antibody

Rabbit Polyclonal
Code

200-401-222
Size

200 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Anti-Bcl-2 is shown to detect Bcl-2 in GP-E86 cell whole cell lysates.  Lanes 1 -10 and 12 are bcl-2 mutants.  Lane 11 is wt bcl-2.  Lane 13 is an empty vector lysate.  Detection occurs using a 1:1,000 dilution of antibody followed by 1:4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.  Film exposure approximately 1’.  Other detection systems will yield similar results.
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Synonyms: Apoptosis regulator Bcl 2 antibody, Apoptosis regulator Bcl2 antibody, AW986256 antibody, B cell CLL/lymphoma 2 antibody, B cell leukemia/lymphoma 2 antibody, B cell lymphoma 2 antibody, Bcl 2 antibody

Bcl-2 Antibody Properties

Description
Anti-Bcl-2 (RABBIT) Antibody - 200-401-222
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, mouse, rat, bovine
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500- 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BCL2
Download CofA
Lot#  

Bcl-2 Antibody Description

Background
Bcl-2 is a human proto-oncogene located chromosome 18.  Its product is an integral membrane protein (also called Bcl-2) located in the membranes of the endoplasmic reticulum (ER), nuclear envelope, and in the outer membranes of the mitochondria.  The gene was discovered as the translocated locus in a B-cell leukemia (hence the name). This translocation is also found in some B-cell lymphomas.  In the cancerous B cells, the portion of chromosome 18 containing the BCL-2 locus has undergone a reciprocal translocation with the portion of chromosome 14 containing the antibody heavy chain locus. This t(14;18) translocation places the BCL-2 gene close to the heavy chain gene enhancer.  This enhancer is very active in B cells and therefore results in high levels of Bcl-2 expression in these cells.  High levels of the Bcl-2 protein protect the cells from early death by apoptosis. The Bcl-2 protein suppresses apoptosis by preventing the activation of the caspases that carry out the process.  It is conceived that introduction of the Bcl-2 gene into the cells of injured tissue will reduce cell death and improve the clinical outcome of the injury
Immunogen
This purified IgG antibody was prepared from rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 62-76 (RDPVARTSPLQTPAA) of human Bcl-2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified IgG antibody against Human Bcl-2 has been tested for use in immunofluorescence microscopy and immunoblotting.  The antibody recognizes a 27 kDa band corresponding to human bcl-2.  Anti-Bcl-2 shows perinuclear staining of Bcl-2.  Reactivity in other immunoassays is unknown.
Purity/Specifity
This purified IgG antibody was prepared from monospecific rabbit antiserum by Protein A chormatography.  The antibody is directed against human Bcl-2 and is useful in determining its presence in various assays.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-381

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry