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Primary Antibodies > Ubiquitin and UBL Antibodies

Ubiquitin Activating Enzyme E1 Antibody

Rabbit Polyclonal

Code

200-401-888

Size

500 µg

Price

$294.00

Availability

Ships next business day.

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Phone: 800.656.7625

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Rockland's Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 µg/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

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Synonyms: A1S9 antibody, A1S9 protein antibody, A1S9T and BN75 temperature sensitivity complementing antibody, A1S9T antibody, A1ST antibody, GXP 1 antibody

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Ubiquitin Activating Enzyme E1 Antibody Properties

Description

Anti-Ubiquitin Activating Enzyme E1 [RABBIT) Antibody - 200-401-888

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:2,000 - 1:10,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 2 mg/ml - 20 µg/ml
Other Dilution: User Optimized

Physical State

Lyophilized

Shipping condition

Ambient

Format

IgG

Label

Unconjugated

Concentration

5.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

100 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

UBA1

Ubiquitin Activating Enzyme E1 Antibody Description

Background

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.

Immunogen

Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This purified antibody is directed against human Ubiquitin Activating Enzyme E1 protein. The product was purified from monospecific antiserum by a multi step procedure. A BLAST analysis was used to suggest that this antibody would react with Ubiquitin Activating Enzyme E1 protein from human (100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E., Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135. Saito,Y., Doi,K., Yamagishi,N., Ishihara,K. and Hatayama,T. (2004) Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems. Biochem. Biophys. Res. Commun. 314 (2), 396-402. Bres,V., et al. (2003) A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter. Nat. Cell Biol. 5 (8), 754-761.

Specific Reference

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