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Primary Antibodies  >  Cancer Research Antibodies
 

SPANX-C Antibody

Rabbit Polyclonal
Code

200-401-915
Size

500 µg
Price

$309.00
Availability

Ships next business day.
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Rockland's affinity purified anti-SPANX-C antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human sperm and spermatids at 60X.  Tissue was formalin-fixed and paraffin embedded.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.  Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Synonyms: Cancer-testis-associated protein CTp11 antibody, CTP11 antibody, Nuclear associated protein SPAN Xc antibody, SPANX family, member C antibody, SPANX family, member C1 antibody

SPANX-C Antibody Properties

Description
Anti-SPANX -C (RABBIT) Antibody - 200-401-915
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1.25-2.5 µg/ml
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
5.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CTP11
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Lot#  

SPANX-C Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm.  Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
This protein A purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue.  Most human cell lines are SPANX negative.
Purity/Specifity
This Protein A purified antibody is directed against human SPANX-C protein.  The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.   A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANX proteins from human, gorilla and chimpanzee.  Reactivity against homologues from is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Kouprina N, Mullokandov M, Rogozin I, Collins K, Solomon G, Risinger J,Koonin E, Barrett JC. Larionov V. (2004) The SPANX gene family of cancer-testis specific antigens: rapid evolution, an unusual case of positive selection and amplification in African great apes and hominids. Proc Natl Acad Sci USA 101: 3077-3082. Westbrook VA, Diekman AB, Klotz KL, Khole VV, von Kap-Herr C, Golden WL, Eddy RL, Shows TB, Stoler MH, Lee CY, Flickinger CJ, Herr JC. (2000)Spermatid-specific expression of the novel X-linked gene product SPAN-X localized to the nucleus of human spermatozoa. Biol Reprod 63: 469-481. Westbrook VA, Schoppee PD, Diekman AB, Klotz KL, Allietta M, Hogan KT, Slingluff CL, Patterson JW, Frierson HF, Irvin WPJr, Flickinger CJ,Coppola MA, Herr JC. (2004)Genomic organization, incidence, and localization of the SPAN-X family of cancer-testis antigens in melanoma tumors and cell lines. Clin Cancer Res 10: 101-112.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-C43

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-C43S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
600-401-A46

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, chimpanzee, macaque
Catalog Number
600-401-A47

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry