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Primary Antibodies  >  Apoptosis Antibodies
 

Survivin Antibody

Rabbit Polyclonal
Code

200-401-A34
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunohistochemical staining using Rockland's IgG Fraction of anti-Survivin antibody at 10 µg/ml shows detection of Survivin in MOLT4 cells. Cells were fixed in 4% paraformaldehyde
Western blot using Rockland's IgG Fraction of anti-Survivin antibody shows detection of a single band at ~17 kDa corresponding to Survivin in MOLT4 cell lysates in the absence (lane A) and presence (lane B) of blocking peptide. Primary antibody was used at 1 µg/ml.
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Synonyms: API4 antibody, Apoptosis Inhibitor 4 antibody, Apoptosis inhibitor survivin antibody, Apoptosis inhibitor4 antibody, Baculoviral IAP repeat containing 5 antibody, Baculoviral IAP repeat containing protein 5 antibody, BIRC 5 antibody

Survivin Antibody Properties

Description
Anti-Survivin (RABBIT) Antibody - 200-401-A34
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
IF Microscopy : 1:100 - 1:1,000
Western Blot : 1:200 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BIRC5
Download CofA
Lot#  

Survivin Antibody Description

Background
Apoptosis, or programmed cell death, is related to many diseases, such as cancer.  Apoptosis is triggered by a variety of stimuli, including members of the TNF family, and prevented by the inhibitor of apoptosis (IAP) proteins.  IAP proteins form a conserved gene family that binds to and inhibits cell death proteases.  A novel IAP protein was recently identified and designated Survivin (also referred to as apoptosis inhibitor 4 (API4) and TIAP).  Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity.  Survivin is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Immunogen
This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human Survivin protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Paraformalde-hyde fixation is recommended for immunohistochemical staining.   Expect a band at ~17 kDa in size corresponding to Survivin by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This IgG fraction antibody is directed against human Survivin protein.  The product was purified from monospecific antiserum by selective precipitation followed by ion exchange chromatography.  Reactivity against Survivin from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, mouse, rat
Catalog Number
600-401-966

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry